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Quantification of steroidogenic acute regulatory gene expression RNA Isolation, cDNA synthesis, and real-time quantitative polymerase chain reaction The homogenate was directly suspended in TRIzol reagent, followed by the extraction of mRNA
23 The Flaming Lips low-budget indie film Christmas On Mars took seven years to complete, with filming starting in 2001 before the movie finally premiered in 2008
Basement Dweller: He is revealed to be an unsympathetic version of this trope in "Moving Bubble Bass", as it is revealed that he lives in his mother's basement
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