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From the obtained cultures, a series of ten-fold dilutions was prepared in a 96-well plate in a volume of 100 l

The analyte detected by SPR may range from large molecule carbohydrates and proteins all the way down to single-particle extracellular vesicles (EVs) (Nguyen et al., 2015

181,182 While research has demonstrated that the transcriptional response to hypoxia in tissues exhibits remarkable time-of-day dependency, which is governed by the endogenous circadian clock within the tissue

The randomization to sequences was stratified by sex and performed dynamically by the minimization technique using the dynamic allocation algorithm, second-best probability set to 15%, with the help of the Medidata-RTSM software