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Platinum-based drugs, such as cisplatin and carboplatin, covalently crosslink DNA through platinum atoms, while cyclophosphamide generates DNA crosslinks through alkylated metabolites, thereby disrupting S-phase replication and inhibiting transcriptional activity, leading to DNA replication stress, cytotoxic effects, and eventual cell death (Goldstein et al., 2008)

smegmatis requires stable and extended contact between a donor and a recipient strain, is DNase resistant, and the transferred DNA is incorporated into the recipient's chromosome by homologous recombination

2 Composition and morphology of bee pollen The BP composition generally depends on plant species, geographical origin, and seasonal conditions