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Description
The entire procedure takes about 10 minutes and there is minimal discomfort during or after the procedure
Electrophoretic mobility shift assay The recombinant proteins GSTECT12 were separately diluted to a series of concentrations ranging from 0 to 2 M in binding buffer (10 mM HEPES-KOH, pH 8.0, 50 mM KCl, 1 mM EDTA, 0.05% (v/v) Triton X-100, 5% (v/v) glycerol, 10 g ml 1 salmon DNA, 1 mM DTT and 40 U ml 1 Ribolock RNase inhibitor (Thermo Fisher Scientific))
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Nicholls SJ, Lincoff AM, Garcia M, Bash D, Ballantyne CM, Barter PJ, et al
