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Next, we examined whether induction of H2A phosphorylation depends on DNA replication

For each condition 1 mL supernatant was collected from the 150 cm 2 flask, centrifuged at 10,000 g and 4 C for 1 min, 500 L were transferred into a 1.5 mL collection tube, snap-frozen in liquid nitrogen, and stored at 80 C until analysis

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Chronic alcohol consumption also is associated with disturbances in the metabolism of sulfur-containing amino acids, leading to increased levels of the amino acids glutamate, aspartate, and homocysteine in alcoholic patients