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Moghaddam SS
KP metabolites can also be used for predicting therapeutic efficacy
pepsin digests with ABC (100 mM), prior to solid-phase extraction (SPE) on 1 cc (30 mg) OASIS HLB cartridges (Waters, Milford, MA) eluted with 100% MeOH (1 mL)
The two values are comparable both from low-receptor-density cAMP assays (Coskun 2022 explicitly used Willard 2020's method), with a small cross-lab offset (~1.2, gauged from native-ligand controls) so treat the ~16 as a ratio rather than an exact multiple
