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In that case, only the highly abundant interference was analyzed in MS2 leading to a MS/MS spectrum of interference fragments and low abundant [M+1+2H] 2+ fragments, not allowing confident identification

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Fluorescence in situ hybridization (FISH) On the first day, brain slices are washed in DEPC-PBS for 5 min, followed by a 5-min wash in DEPC-PTW (0.1% Tween-20 in PBS), and then incubated in 2 SSC + 0.5% Triton -100 for 30 min