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EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell Culture and Transfection Immortalized human mammary epithelial cells HMEC-hTERT1 cells were cultured in MEGM Mammary Epithelial Cell Growth Medium (Lonza CC-3150) (Sack et al., 2018)

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Clinically, DN is characterized by sensory deficits, such as tingling, numbness, and allodynia, along with motor impairments, including weakness and loss of coordination (Callaghan et al., 2012)