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To determine citrate synthase activity, the rate of coenzyme A (CoA.SH) production from oxaloacetate was measured by quantifying the levels of free sulfhydryl groups using the thiol reagent DTNB

They will conduct a thorough physical examination to assess any visible signs of allergic reactions such as swelling or skin rashes, and skin prick tests may also be performed with hydroxocobalamin or related substances to determine allergic sensitivities

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