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To obtain information on the thermodynamics of the calcium affinity to alpha-synuclein, and as the number of calcium cations that are bound to alpha-synuclein was not known, we performed a global analysis by fitting the chemical shift perturbation using a multiple ligand model 17 , which allows to estimate the binding constant when the exact number of ligands is not known

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Long-range regulation of p53 DNA binding by its intrinsically disordered N-terminal transactivation domain