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Description
coli Rosetta2(DE3)pLacI cells harbouring the pET22b expression plasmid were grown in YT media at 298 K to an OD 600 of 0.6

Absence of expected improvement by mid-protocol (4-6 weeks) warrants reassessment

[DOI] [PubMed] [Google Scholar] 103.Redon R., Ishikawa S., Fitch K.R., Feuk L., Perry G.H., Andrews T.D., Fiegler H., Shapero M.H., Carson A.R., Chen W., et al
van Leyen K, Kim HY, Lee SR, Jin G, Arai K, Lo EH
