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Proteins were separated by SDSPAGE, transferred to PVDF membranes (Millipore) and probed with primary antibodies in 1 TBST buffer (20 mM Tris pH 7.5, 150 mM NaCl and 0.1% Tween-20) with 5% fat-free milk at 4 C overnight

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Their activity is primarily linked to their ability to cross-link DNA strands

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doi: 10.1016/j.neulet.2005.12.067

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In a study of BO patients, 359 all subjects exhibited a smaller respiratory community dominated by Aspergillus , and bacterial diversity was temporarily reduced with BO and correlated with neutropenia and antibiotic treatment

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