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Description
Transient transfection of HEK293T cells was performed using Fugene6 (Promega) according to the manufacturers instructions
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Additional risk factors include: Age over 60 years (reduced stomach acid production) Autoimmune conditions (thyroid disease, vitiligo, type 1 diabetes) Family history of pernicious anaemia Chronic alcohol consumption Diagnosis and Testing Diagnosis of vitamin B12 deficiency anaemia requires a combination of clinical assessment, laboratory investigations, and sometimes specialist testing
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