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Then brain sections were incubated with rabbit monoclonal anti-HDAC9 antibody (Abcam, #ab109446, 1:200) in combination with one of following antibodies: mouse monoclonal anti-NeuN antibody (MilliporeSigma, #MAB377, 1:200), goat polyclonal anti-IBA1antibody (Abcam, #ab5076, 1:200) or mouse monoclonal anti-GFAP antibody (cell signaling technology, #3670, 1:200) diluted in TBST containing 10% donkey serum overnight at 4 C
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It included comparing four treatment options against the standard of care to evaluate their effectiveness against Coronavirus

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