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Plasmids and constructs Single guide RNAs (sgRNAs, Supplementary Table 2) were designed using the VBC score sgRNA prediction tool 82 and cloned into a lentiviral vector enabling expression of the sgRNA and IRFP670 (pLenti-hU6-sgRNA-PGK-IRFP670)
Long-term treatment of PD with Mucuna pruriens seed powder is more effective than treatment with conventional LD

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Larsen, 2003)