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Jing X, Yang F, Shao C, Wei K, Xie M, Shen H, et al

doi:10.1016/j.jneuroim.2014.05.005 Sahin, Azad Gazi et al

The recombinant proteins were extracted with ice-cold lysis buffer containing 20 mM Tris-HCl (pH 7.4), 0.5% Triton X-100, 150 mM NaCl and 2 mM EDTA and then were affinity purified using glutathione Sepharose 4B beads (GE healthcare, Chicago, IL, USA)

The activity of these enzymes is enhanced by a variety of chemicals, including caffeine[40, 41] and anticonvulsant drugs[42], and it is well known that co-ingestion of these drugs with APAP can greatly enhance the toxicity of APAP
