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P19 Cell Differentiation To induce neuronal differentiation, the stably transfected P19 cells were cultured in bacteriological-grade Petri dishes at a seeding density of 1 10 5 cells/ml in the presence of 1 M all-trans-retinoic acid (Sigma-Aldrich, USA) in minimum essential medium (Wako, Japan) supplemented with 10% fetal bovine serum (Sigma-Aldrich, USA) 38
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