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All of the studies used both in vivo and some form of in vitro methods of data collection in order to observe different treatment effects, allowing for biomechanical, functional (use of AFI index and load to failure), macroscopic and microscopic histological analysis

Oxidative stress caused by physical/emotional stress, strenuous exercise, exposure to environmental toxins, and immune stress can all cause an increase in free radicals, which may cause oxidative stress and deplete glutathione

Conversely, advanced systems, such as brain organoids, humanized mice and large animal models, more closely mimic human pathology but come with higher costs, complexity and interpretative challenges 37