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Description
The supernatant containing the proteasomes were separated after centrifugation at 12,000 xg for 15 min at 4 C and were quantified and diluted to 1 g/L concentration with proteasome lysis buffer
Neuroscience lett
To achieve better surface expression, the native signal peptide of GluK3 was replaced with that of GluK2 and was sub-cloned into the pEGBacMam vector 48 for baculovirus-based expression in mammalian cells using standard molecular biology techniques
Epigenet Chromatin 18(1):4
