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Preparation of protein lysates The cells collected from 6-well plates were lysed in 0.4 ml lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 2% SDS) followed by sonication (10 cycles of 10 s pulses with 10 s rest intervals) using a Fisher Scientific Sonic Dismembrator Model 500 at 15% amplitude

Once they get used to you "fooling" with their ears, a lot of them will actually come running when you get the testing supplies out (because they want their special treat!) Plain old chicken, freeze dried chicken (or any other protein source) are good treats for a diabetic cat #29 is it common to cut their nails during blood curves, or when you take them up for a blood curve testing