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Single-molecule-based approaches, such as subunit stoichiometry determination via TIRF microscopy, single-molecule FRET, both based on fluorescence microscopy, and mass photometry, which does not require the presence of fluorophores (discussed in the next section), have become useful tools for the investigation of oligomerization in the membrane environment [135]

If pain is relieved from an anesthesia injection it is indicative that the injury is occurring inside the joint, rather than somewhere outside of it

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Sometimes, the most significant breakthroughs come from refining what we already have, making established molecules more robust, more reliable, and more effective for study