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Highly expressed DEGs acted on the GSSG-GSH cycle and AsA-GSH cycle in glutathione metabolism (Fig
Furthermore, miRNAs have potential applications in genetic engineering, where their manipulation can improve crop quality and create hybrids with enhanced heat tolerance (Zheng et al., 2025
Side effects of glutathione: what to watch for Most people handle glutathione well, and clinical trials show few side effects

The real-time PCR procedure was set up as follow, 2 l cDNA (20 ng), 0.4 l of each primer (200 nM), 10 l SYBR green master mix (Thermofisher Scientific) and nuclease-free water to bring the volume to 20 l
