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However, there is evidence linking abnormalities in thiamine availability and metabolism to the pathophysiology of AD

R., & Jones, M

Spheroid-forming assays DMEM-F12 medium without serum, supplemented with 0.4% bovine serum albumin, B27 (50 dilution, Invitrogen), 20 ng/mL basic fibroblast growth factor, 10 ng/mL epidermal growth factor, and 5 g/mL insulin (Invitrogen) was used to plate 5000 cells per well in six-well ultralow attachment plates

A growing body of evidence suggests that gut microbiota is involved in the digestion and utilization of fibers such as polysaccharides, which otherwise cannot be utilized by the host