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It is characterised by genetic and autoimmune components, with hyperactivation of the immune system and an inflammatory cycle that self-sustains developing around the TNF/IL-23/IL-17 axis [47]

Freezing and thawing a reconstituted peptide can cause the formation of ice crystals that, on a microscopic level, can shear and degrade the peptide chains

40 cycles of 98 C for 15 s, 60 C for 20 s, and 72 C for 30 s
