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S2: Centrifugation to collect the supernatant as crude enzyme solution

Nov 2021;75(11):1540-1554

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In this experiment, the average lifespan of the nhr-80 mutant was 12.5 0.5 d as compared to 13.9 0.4 d in wild-type animals and 8.2 0.2 d in nhr-49 mutants when grown at 25 C