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For experiments involving oxygen control, purified CD8 + T cells were immediately acclimatized to the required oxygen tension (21%, 5% or 1% O 2 ) for 2 h in complete RPMI 1640 supplemented with 10% FBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, before activation with anti-CD3/CD28 Dynabeads and 30 U ml 1 IL-2

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[DOI] [PMC free article] [PubMed] [Google Scholar] 19.Andr S., Picard M., Cezar R., Roux-Dalvai F., Alleaume-Butaux A., Soundaramourty C., Cruz A.S., Mendes-Frias A., Gotti C., Leclercq M., et al

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