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Reagents and Equipment Required but Not Provided: Reagents and Equipment Required but Not Provided: Spectrophotometer, balance, table centrifuge, 1 mL glass cuvette, mortar/homogenizer, EP tube

In the Se-poor belt in China which ranges from north-east to south-west, Se status is decisively affected by monsoonal precipitation (Reference Blazina, Sun and Voegelin8)

Among these, the VacA toxin forms anion channels on the host cell membrane, disrupting mitochondrial membrane potential and inducing cytoplasmic vacuolization, ultimately leading to cell death [29]

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