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Description
Excess media was removed and replaced with 70% L15 media containing 10 M DCFH-DA and incubated at 20C for 30 min (32), after which the solution was removed, replaced with 10 g/ml Calcofluor White (CFW) to stain Bd for 5 mins (28), rinsed and replaced with 70% L15 media
10.1038/s41598-020-80864-3
depending on the residual drug levels at the active site, they also may competitively inhibit metabolism of a simultaneously administered drug
Consequently, energy metabolism is affected, resulting in progressive motor dysfunction
