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*Correspondence: Nayeem Ahmad, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

If necessary, the eluted protein can be concentrated using ultrafiltration or dialysis against an appropriate buffer to remove excess glutathione and prepare the protein for downstream applications

The whole procedure was conducted under cold conditions

Therefore, there is an attempt to induce methyl deficiency through feed additives, such as guanidinoacetic acid (GAA), to better evaluate the role of RPM in supporting methyl donor supply