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Subsequently, the sections were incubated with secondary antibody (goat anti-rabbit), rinsed with phosphate buffer saline (PBS), stained with DAB, and hematoxylin and eosin (H&E) staining, dehydrated, transparentized, and mounted before microscopic analysis
Note: A 4.5 mg weekly dose draws about 45 units (0.45 mL) roughly half the reconstituted vial

Well, its simply because we want the pH expressed in positive numbers

Infections related to vaccine injection sites are exceedingly rare
