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doi: 10.1038/cddis.2013.84 Valentijn LJ, Koster J, Haneveld F, Aissa RA, van Sluis P, Broekmans ME, Molenaar JJ, van Nes J, Versteeg R ( 2012 )
The total RNA of the cells was extracted and reverse transcribed into cDNA, and the expression of pro-inflammatory cytokines in the cells was analyzed by real-time reverse transcription-polymerase chain reaction (RT-PCR) using SYBR Green/ROX qPCR Master Mix (Thermo Scientific), and the sequence of primers is listed in Additional file 1: Table S1
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This adds another layer of complexity to the immune-tumor interaction in platinum resistance
