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To obtain pgfp, a gfp containing fragment was released from pUCG-H [34] via Sma I and Sac I and subcloned into the corresponding Eco RV and Sac I sites of pGEM5zf+ (Promega)

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Typical protocols: Standard research protocols use 2-2.5mg of TB-500 twice weekly during an initial loading phase, typically lasting 4-6 weeks
