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By employing whole-genome haploid screening and CRISPR-Cas9 technology, two pivotal drivers of ferroptosis were identified: Acyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 (LPCAT3), both of which function as membrane remodeling enzymes [75,76,77]

doi: 10.2310/JIM.0b013e3181d88fde

After the baseline, three treatments of either NAC (600 mg/kg) or saline (12 mL/kg) intraperitoneal (i.p.) injections were applied (randomly assigned as Treatment 1 or Treatment 2) at ZT22, ZT0, and ZT3 (Figure 2A)

MSC-Exos activate Nrf2/HO-1 expression via the GOT1/CCR2 signaling pathway to prevent ferroptosis, reduce inflammation in OA mice, and improve their performance, providing a potential treatment for OA and other orthopedic diseases