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These systemic changes were functionally validated in vitro: GCLC knockdown in rat astrocytes led to elevated ROS accumulation, mitochondrial membrane depolarization, and increased apoptosis

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In this study, an increase in myo-inositol level was observed in pesticide-treated worms compared to control worms, indicating disturbances in the metabolism of phosphatidylinositol phosphate upon exposure to selected pesticides suggesting myo-inositol as a sensitive biomarker for assessing pesticide toxicity in earthworms, similar results were reported by 26,54