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Description
The supernatant (cytosolic proteins) was collected, and the pellet (membrane proteins) was resuspended in RIPA buffer with detergents and centrifuged at 16,000 g for 45 min at 4 C
The development of recombinant DNA technologies, synthetic biology and metabolic engineering, alongside biosafety and high production yields, has enhanced the value of certain yeasts as cell factories for production [2]
Trimethyltin-induced differential expression of PAR subtypes in reactive astrocytes of the rat hippocampus
doi: 10.1371/journal.pone.0157824
