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Concentrate the cleaved unbound protein to a final volume that is 0.5 to 1% of the column volume to be used for the separation using an ultrafiltration device, such as Amicon Ultra (Millipore), according to the manufacturers instructions (see Note 37)

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The chronic complications of type 2 diabetes affect several organs and tissues, including the eyes, kidneys, heart, blood vessels, and peripheral nerves

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