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The catalytic mechanism of GSTZs differs from other GSTs in that GSH is initially conjugated to the cis double bond of maleylacetoacetate allowing the isomerisation reaction, before being eliminated in a second step allowing the formation of fumarylacetoacetate (Thom et al., 2001)

Glutathione synthase catalyzes the biosynthesis of glutathione (GSH) which is the major component of GST activity [1]

Therefore, the sampling time with a 1-h interval may not be enough to reflect the GH release pattern of mice compared with a 10-min interval routinely used in this laboratory (Steyn et al., 2011

Bell, MD, FACP By Jon Johnson on July 20, 2020, viewed on December 28, 2022