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Another risk factor for APAP toxicity is ethanol

The truncation reduces IGFBP binding similar to the LR3 modification, but DES IGF-1 retains a much shorter circulating half-life (typically reported in the 20 to 30 minute range) because the rest of the molecule remains susceptible to standard proteolytic clearance

In particular, l-glutamate is able to displace the quenched rhodamine indicator from the cage, whose fluorescence is then fully restored

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