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Membranes were blocked with 5% bovine serum albumin (Sigma, A3912-100g) and incubated overnight at 4C with primary antibodies [ GAPDH (Santa Cruz, sc-47724), cleaved PARP (CST, 5625S), cleaved caspase 3 (CST, 9661S), cleaved caspase 7 (CST, 9491S), HMOX-1 (CST, 43966S), GCLM (Abcam, ab126704), NQO1 (CST, 3187S), NRF2 (CST, 12721S), KEAP1 (CST, 8047S), PRDX1 (CST, 8499S), GSTO1 (Thermo, PA5-83383)] in 5% BSA in TBS-T (Bio-Rad, 170-6435)

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