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mortality: 6.9%), followed by lung (morbidity: 11.4%

Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT

The inhibition of complex I activity can further promote the abnormal accumulation of -syn, creating a vicious cycle (Betarbet et al., 2000)

doi: 10.1016/j.jns.2010.11.018 159 YoshiuchiKFarkasJNatelsonBH