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The half-maximal inhibitory concentration (IC 50 ) of RSL3 AML12 cells were treated with 0, 5, 10, 20, 30, 40, and 50 mM RSL3 for 24 h, then the cell inhibition rate was determined by CCK8 assay

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On the other hand, PAM is a bifunctional enzyme involved in the synthesis of hormones and neurotransmitters such as oxytocin, calcitonin, vasopressin, neuropeptide Y, GLP-1, and substance P, found in tissues such as the pituitary and adrenal glands