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A dilution series of 05 mM GSH, together with 2.5 mM CDNB (from a 62.5 mM stock solution in ethanol), in GST assay buffer (25 L/well) was mixed with an equal volume of the protein solution (1 M GST, 2.5 mM CDNB in GST assay buffer) in a 96-well flat bottom microtest plate (Sarstedt, Nmbrecht, Germany) at 30 C and the absorbance at 352 nm was measured every 20 s for 10 min in a Synergy 2 microtiter plate reader (BioTek, Winooski, VT) using Gen5 version 1.9 software (BioTek)

doi: 10.1016/j.cell.2024.02.005 140 XiaYYangQLiQWenJLiMWuZet al

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