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Bone mineral density reduction

The reactions were stopped by adding 5 protein sample buffer (250 mM Tris-HCl pH 6.8, 10% SDS, 50% glycerol, 0.5% bromophenol blue and 5% -mercaptoethanol), and the samples were boiled for 5 min before separation by SDSPAGE and immunoblotting

Muscle-protective effects of Schisandrae Fructus extracts in old mice after chronic forced exercise

Purbia et al
