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Cartilage research employing AOD-9604 often uses ex vivo tissue explants or primary chondrocyte cultures, with outcomes measured through histological staining (safranin-O, toluidine blue) and proteoglycan quantification

TLR4 binds LPS from Gram-negative bacteria, which causes proinflammatory cytokine release (TNF, IL-1, IL-6) without degranulation (23, 24)

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