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Description
Proteins in liver tissues or cell lysates were electrophoretically separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred to a nitrocellulose membrane, and blocked with skimmed milk powder and incubated at 4 C overnight with primary antibodies

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44 In this approach, nitrate (NO 3 ) reacts with sulfanilamide and N-(1-naphthyl)ethylenediamine dihydrochloride to produce a colored product, with maximum absorbance at 490 nm

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