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K.AntonelliP
Preparation Condition 3 (EDTA 80 o C deproteinization): Deproteinization may not be necessary to prevent spontaneous oxidation of free GSH Unexpectedly, we found no difference in the levels of total GSH, free GSH, GSSG, or the ratio of free GSH to GSSG between the EDTA-treated plasma samples which were stored at 80 o C (Preparation Condition 2 and Preparation Condition 3)
HCC tumorigenesis The accumulation of ROS drives the malignant transformation of liver fibrosis and cirrhosis toward HCC, also having a significant impact in the initiation of HCC [85]
Vol L, p.61
