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The tissues were counterstained with DAPI (4,6-diamidino-2-phenylindole) for 10 min at room temperature to label the nuclei and then mounted on slides with anti-fade medium (ProLong Gold, Thermo Fisher Scientific Inc., Rockford, IL)

To ten sam compound acetylowany Epithalon (Ac-AEDG)
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The benefits of using whole tissue vaccines are that it contains relevant tumor antigens (targets for the immune system) and can be produced without knowing the specific antigens of your pets tumor
