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Description
coli Rosetta (DE3) cells in auto-induction Terrific Broth (TB) supplemented with 50 g/ml kanamycin, 34 g/ml chloramphenicol, 0.3 mM -aminolevulinic acid, 0.0025% pyridoxineHCl, 0.001% thiamineHCl, and 0.1 mM ferric chloride at 30 C, 200 rpm for 24 h
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