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In addition, we were able to resolve the interaction sites at the single amino acid level

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Cells were loaded with 20 M ThiolTracker Violet glutathione detection reagent and 100 nM SYTO 16 Green Fluorescent Nucleic Acid Stain (Invitrogen, catalog # S7578) and imaged within the hypoxic environment using an LS620 microscope (Etaluma, Carlsbad, CA, USA) fitted with a 20 objective at 0, 30 min, and 6 h at 1% O 2