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To prevent cross-contamination, cell lysis for protein extraction was performed as in the RNA isolation method

doi: 10.1016/j.tem.2010.01.009 [DOI] [PMC free article] [PubMed] [Google Scholar] 23.Donath MY

To determine whether mere exposure to IL-1 is sufficient to generate a pro-arrhythmogenic substrate, ventricular myocytes were incubated for 24 h with IL-1 and the number of spontaneous contractile events (NSE), an index of cellular arrhythmic activity, was measured after pacing the cells during 10 min at either 0.5 or 3 Hz

Zorrilla E, Della Pietra A, Russo AF
